Have questions?

Purigen Biosystems, Inc.

Ionic® Purification Kits

Fluidic chips and reagents for the Ionic® Purification System

GET QUOTE    VIEW BROCHURE

Ionic Kits by Application

Ionic Purification Kits and protocols support automated purification of specific sample types and are required for use with the Ionic Purification System. Purification protocols are developed and validated at Purigen Biosystems to maximize purification yield and quality with minimal manual steps.

Ionic® Cells to Pure DNA Kit
Ionic Cells to Pure DNA Kit

Each Cells to Pure DNA Kit contains 6 Ionic Fluidic Chips and reagents to purify DNA from 48 samples.


Sample

Sample type Cultured cells
Sample preps per kit 48
Sample preps per chip 8

Density

Maximum cell density per sample 100,000 cells (diploid equivalents)
Minimum cell density per sample 1,000 cells (diploid equivalents)

Volume

Input volume per sample prep ~210 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.2 mL
Lysis Buffer 2 1 bottle; 7.2 mL
RNAse Reagent 1 bottle; 100.0 µL
Proteinase K Reagent 1 bottle; 150.0 µL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 1 bottle; 18.0 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL

Download Product Info Sheet

Go to Application Page



Ionic® FFPE to Pure DNA Kit
Ionic FFPE to Pure DNA Kit

Each FFPE to Pure DNA Kit contains 6 Ionic Fluidic Chips reagents to purify DNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 4
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 6 bottles; 1.8 mL
DNAse Reagent 1 bottle; 250.0 µL
Proteinase K Reagent 1 bottle; 480 µL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 15.0 mL

Download Product Info Sheet

Go to Application Page


Ionic® FFPE to Pure RNA Kit
Ionic FFPE to Pure RNA Kit

Each FFPE to Pure RNA Kit contains 6 Ionic Fluidic Chips and reagents to purify RNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 2
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Elution volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 6 qty
Lysis Buffer 1 6 bottles; 1.4 mL
Lysis Buffer 2 1 bottle; 4.0 mL
DNAse I Reagent 1 bottle; 600.0 µL
Proteinase K Reagent 1 bottle; 1.0 mL
Extraction Buffer 1 bottle; 12.0 mL
Anodic Buffer 1 bottle; 12.0 mL
Separation Buffer 6 bottles; 2.5 mL
Neutralization Buffer 1 bottle; 12.0 mL
Cathodic Buffer 1 bottle; 12.0 mL
Mineral Oil 1 bottle; 20.0 mL

Download Product Info Sheet

Go to Application Page


Ionic® FFPE Complete Kit
Ionic FFPE to Pure RNA Kit

Each FFPE Complete Kit contains 12 Ionic Fluidic Chips and reagents to purify RNA and DNA from 48 samples.


Sample

Sample type FFPE scroll / tissue section
Sample preps per kit 48 (RNA and DNA from each)
Sample preps per chip 8

Cross-section

Maximum number of 10 µm thick scrolls/curls 2
Recommended cross-sectional area per scroll/curl 50 mm2 – 300 mm2

Volume

Input volume per sample prep ~200 µL of lysate
Extract volume per sample prep ~40 µL

Run-time

Run-time per chip ~60 mins

Contents

Fluidic Chip 12 qty
Lysis Buffer 1 12 bottles; 1.4 mL
Lysis Buffer 2 1 bottle; 7.0 mL
DNase I Reagent 1 bottle; 700.0 µL
RNase Reagent 1 bottle; 700.0 µL
Proteinase K Reagent 1 bottle; 1.5 mL
Extraction Buffer 1 bottle; 24.0 mL
Anodic Buffer 1 bottle; 24.0 mL
Separation Buffer 2 bottles; 15 mL
Neutralization Buffer 1 bottle; 24.0 mL
Cathodic Buffer 1 bottle; 24.0 mL
Mineral Oil 1 bottle; 20.0 mL

Download Product Info Sheet

Go to Application Page


Ionic Reagents and Kit

 

Purigen Isotachophoresis Technology

Isotachophoresis separates and concentrates charged molecules in solution solely based on their electrophoretic mobility. Biological samples are gently lysed and added to the Purigen Ionic® Fluidic Chip which applies an electric field to isolate nucleic acid in its natural, native form.